Neuronal P2X7 receptor-induced reactive oxygen species production contributes to nociceptive behavior in mice

Neuronal P2X7 receptor-induced reactive oxygen species production contributes to nociceptive behavior in mice

Intrathecal administration of BzATP resulted in ROS production in the spinal cord and oxidative DNA damage in dorsal horn neurons.
To confirm CellROX intensity increased due to ROS production, cells were pre-treated with the ROS scavenger N-tert-butyl-α-phenylnitrone (PBN, 30 µM) for 30 min.
ATP induces ROS increase in spinal cord dorsal horn (SCDH) neurons.
About 79% of neurons showed ROS increase to BzATP (300 µM) treatment after 20 min (33 of 42 neurons). When we pre-treated neurons with A438079 (A43) and co-treated with BzATP (300 µM), ROS production was completely blocked (Fig.
P2X7R activation with BzATP induces ROS production in SCDH neurons.
(A) Representative of live cell confocal images captured before (0 min) and after application of BzATP in the presence of vehicle or apocynin (100 µM, 250 µM).
These results indicate that P2X7R activation increases ROS production in the SCDH of mice.
Spontaneous nociceptive behavior was measured every 2 min for 16 min.

Unlikely Partners in Pain App Study

Unlikely Partners in Pain App Study

Purdue Pharma and a Pennsylvania-based healthcare provider have announced the enrollment of their first patient in a joint study of wearable health technology. As many as 240 people will eventually be enrolled in the two-year study, which is designed to see if “wearables” can help manage chronic pain.
Geisinger patients enrolled in the study will get an Apple Watch and iPhone equipped with pain apps that will measure their physical activity, self-reported pain, disability, sleep quality, depression, medication use and heart rate.
Patients who report pain will be prompted to try non-pharmaceutical alternative therapies, such as stretching, mindfulness and thermotherapy.
“The goal of this technology is to improve patient function and quality of life while reducing the need for analgesic medications.
The proposed multi-level integrated platform will facilitate and accelerate the speed of communication between the patient and healthcare providers, thereby allowing quicker patient access to appropriate care,” said John Han, MD, director of Pain Medicine at Geisinger.
The study comes as Purdue fights a seemingly endless series of court battles with state and local governments over its marketing of OxyContin over a decade ago. Critics contend the overprescribing and abuse of OxyContin helped launch the overdose crisis.
A recent study by Geisinger found that opioids are ineffective in treating chronic pain and increase the risk of overdose and death.

Plantar Fasciitis: Overcoming Heel and Arch Pain Naturally

Plantar Fasciitis: Overcoming Heel and Arch Pain Naturally

One of the most common foot maladies people suffer from is plantar fasciitis.
Like most people, doctors typically think of the foot as somehow separate from the rest of the body–they focus on treating only the foot and don’t look at the other physiological systems or diet and lifestyle factors that go into creating these problems in the first place.
If you want to heal a chronic foot problem, you need to treat your whole body.
So, my job as a holistic podiatrist is to stimulate the pathway to healing (primary inflammation) and block the pathway to plantar fasciitis and other forms of foot pain (chronic inflammation).
The most common treatment for the condition is cortisone, a powerful steroid that blocks inflammation.
Treating Your Feet from the Ground Up: Diet/Lifestyle Changes to Heal Plantar Fascitiis There are two factors we need to consider when treating plantar fasciitis.
The second is identifying burdens on the immune system that make primary inflammation less efficient.
Proper hydration facilitates cellular repair, and most people don’t get enough water.
The goal is to work with, not against, the immune system so we can effect a complete healing.
I have been treating patients who come to see me with plantar fasciitis using the steps above for over 30 years.

‘DWTS’: Lindsey Stirling Brought to Tears by Painful Rib Injury, Powers Through for Epic ‘Halloween’ Dance

‘DWTS’: Lindsey Stirling Brought to Tears by Painful Rib Injury, Powers Through for Epic ‘Halloween’ Dance

Lindsey Stirling powered through some intense pain on Monday’s Dancing With the Stars to deliver a spooky, stunning performance for the season’s Halloween Night.
Stirling and her partner Mark Ballas dominated last week’s DWTS, earning a perfect score for their Argentine tango and coming in at the top of the leaderboard. “I have to do things full out if I’m gonna know what it feels like to do them in the performance, and yet I’ve got this rib that I feel in pretty much every movement,” Stirling explained. “I came in early today so I could rehearse, because I don’t feel ready, and I can’t even rehearse because I’m in so much pain,” she told Ballas as she cried in the rehearsal studio. “I’m in pain.
I’m not gonna lie,” she told co-host Tom Bergeron, breathing hard.
After getting the judges’ feedback, Bergeron addressed Ballas about how he tried to get Stirling to take things easy during the week.
However, even though the first dance of the night was so hard on her ribs, Stirling acknowledged that she still had the group dance to nail later in the episode.
Stirling managed to power through the pain once again and the group earned a 24 out of 30.
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More surgical mesh injuries denied ACC cover

More surgical mesh injuries denied ACC cover

A review of mesh injury claims from 2005 to 2017 showed one in five claims were declined.
* Using surgical mesh for hernias ‘safe and effective’, surgeon says * ‘Soul destroying’ meeting with Health Minister, surgical mesh victim advocates say * Surgical mesh victim says patients have been betrayed * Four mesh deaths: ‘Mum always put on a brave face for us’ * Hernia mesh complication ‘torture’ for grandfather Spod’s nightmare started six years ago with a hernia repair procedure.
The mesh used to reinforce his weak abdominal wall now sits on a shelf at his home in Edgecumbe, Bay of Plenty, shrivelled up into a rust-coloured lump with sharp wiry edges.
A claim to ACC was declined. “The surgeon said the ruling is that everybody has pain after an operation and it is just not really tangible or measurable, pain is not a condition that ACC accepts,” Spod said.
During another surgery a few months later the surgeon found the hernia mesh shrivelled up near his bladder and removed it. “No-one disputes that Mr Spod is in pain, but ACC cannot cover pain without an identified physical injury and the external opinions provided to ACC could not identify any injury causing his pain.”
The New Zealand Association of General Surgeons said chronic pain could happen in less than 10 per cent of hernia surgery patients.
Actual figures are unknown in New Zealand as there is no registry for mesh and mesh complications. “Some surgeons say yes ‘this is an injury, it is this nerve and here is where it is damaged’ and others don’t – that is why some claims are approved and some aren’t.”